Cerniauskas E, Kurzawa-Akanbi M, Xie L, Hallam D, Moya Molina M, White K, Steel D, Doherty M, Whitfield P, Al-Aama J, Armstrong L, Kavanagh D, Lambris JD, Korolchuk V, Harris C and Lako M. Complement modulation reverses pathology in Y402H-retinal pigment epithelium cell model of AMD by restoring lysosomal function. Stem Cells Transl Med 2020 Dec;9(12):1585-1603.

Collin J, Queen R, Zerti D, Dorgau B, Georgiou M, Djidrovski I, Hussain R, Coxhead JM, Joseph A, Rooney P, Lisgo S, Figueiredo F, Armstrong L, Lako M. Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface. Ocul Surf. 2020 Jun 3:S1542-0124(20)30097-5.

Sungnak W, Huang N, Bécavin C, Berg M, Queen R, Litvinukova M, Talavera-López C, Maatz H, Reichart D, Sampaziotis F, Worlock KB, Yoshida M, Barnes JL; HCA Lung Biological Network. SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes. Nat Med. 2020 May;26(5):681-687.

Georgiou M, Chichagova V, Hilgen G, Dorgau B, Sernagor E, Armstrong L, Lako M. Room temperature shipment does not affect the biological activity of pluripotent stem cell-derived retinal organoids. PLoS One. 2020 Jun 1;15(6):e0233860.

Brydon EM, Bronstein R, Buskin A, Lako M, Pierce EA, Fernandez-Godino R. AAV-Mediated Gene Augmentation Therapy Restores Critical Functions in Mutant PRPF31+/- iPSC-Derived RPE Cells. Mol Ther Methods Clin Dev. 2019 Nov 11;15:392-402.

Ghareeb AE, Lako M, Steel DH. Coculture techniques for modeling retinal development and disease, and enabling regenerative medicine. Stem Cells Transl Med. 2020 Aug 7. doi: 10.1002/sctm.20-0201.

Tzoumas N, Hallam D, Harris CL, Lako M, Kavanagh D, Steel DHW. Revisiting the role of factor H in age-related macular degeneration: Insights from complement-mediated renal disease and rare genetic variants. Surv Ophthalmol. 2020 Nov 4:S0039-6257(20)30146-6. doi: 10.1016/j.survophthal.2020.10.008. Online ahead of print. PMID: 33157112.

Zerti D, Molina MM, Dorgau B, Mearns S, Bauer R, Al-Aama J and Lako M. IGFBPs mediate IGF-1’s functions in retinal lamination and photoreceptor development during pluripotent stem cell differentiation to retinal organoids. Stem Cells (in press).

Understanding the complexity of retina and pluripotent stem cell derived retinal organoids with single cell RNA sequencing: current progress, remaining challenges and future prospective. Zerti D, Collin J, Queen R, Cockell SJ, Lako M. Curr Eye Res. 2019 Dec 3. doi: 10.1080/02713683.2019.1697453. [Epub ahead of print] PMID: 31794277

Quantification of the morphological characteristics of hESC colonies. Orozco-Fuentes S, Neganova I, Wadkin LE, Baggaley AW, Barrio RA, Lako M, Shukurov A, Parker NG. Sci Rep. 2019 Nov 26;9(1):17569. doi: 10.1038/s41598-019-53719-9. PMID: 31772193

Human iPSC differentiation to retinal organoids in response to IGF1 and BMP4 activation is line- and method-dependent. Chichagova V, Hilgen G, Ghareeb A, Georgiou M, Carter M, Sernagor E, Lako M, Armstrong L. Stem Cells. 2019 Nov 13. doi: 10.1002/stem.3116. [Epub ahead of print] PMID: 31721366

Developing a simple method to enhance the generation of cone and rod photoreceptors in pluripotent stem cell-derived retinal organoids. Zerti D, Dorgau B, Felemban M, Ghareeb AE, Yu M, Ding Y, Krasnogor N, Lako M. Stem Cells. 2019 Oct 31. doi: 10.1002/stem.3082. [Epub ahead of print] PMID: 31670434

Seeding hESCs to achieve optimal colony clonality. Wadkin LE, Orozco-Fuentes S, Neganova I, Bojic S, Laude A, Lako M, Parker NG, Shukurov A. Sci Rep. 2019 Oct 25;9(1):15299. doi: 10.1038/s41598-019-51897-0. PMID: 31653933

Differentiation of Retinal Organoids from Human Pluripotent Stem Cells. Chichagova V, Dorgau B, Felemban M, Georgiou M, Armstrong L, Lako M. Curr Protoc Stem Cell Biol. 2019 Sep;50(1):e95. doi: 10.1002/cpsc.95. PMID: 31479596

Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences. Mellough CB, Collin J, Queen R, Hilgen G, Dorgau B, Zerti D, Felemban M, White K, Sernagor E, Lako M. Stem Cells Transl Med. 2019 Jul;8(7):694-706. doi: 10.1002/sctm.18-0267. Epub 2019 Mar 27. PMID: 30916455

Decellularised extracellular matrix-derived peptides from neural retina and retinal pigment epithelium enhance the expression of synaptic markers and light responsiveness of human pluripotent stem cell derived retinal organoids. Dorgau B, Felemban M, Hilgen G, Kiening M, Zerti D, Hunt NC, Doherty M, Whitfield P, Hallam D, White K, Ding Y, Krasnogor N, Al-Aama J, Asfour HZ, Sernagor E, Lako M. Biomaterials. 2019 Apr;199:63-75. doi: 10.1016/j.biomaterials.2019.01.028. Epub 2019 Jan 22. PMID: 30738336

An integrated transcriptional analysis of the developing human retina. Mellough CB, Bauer R, Collin J, Dorgau B, Zerti D, Dolan DWP, Jones CM, Izuogu OG, Yu M, Hallam D, Steyn JS, White K, Steel DH, Santibanez-Koref M, Elliott DJ, Jackson MS, Lindsay S, Grellscheid S, Lako M. Development. 2019 Jan 29;146(2). pii: dev169474. doi: 10.1242/dev.169474. PMID: 30696714

CRX Expression in Pluripotent Stem Cell-Derived Photoreceptors Marks a Transplantable Subpopulation of Early Cones. Collin J, Zerti D, Queen R, Santos-Ferreira T, Bauer R, Coxhead J, Hussain R, Steel D, Mellough C, Ader M, Sernagor E, Armstrong L, Lako M. Stem Cells. 2019 May;37(5):609-622. doi: 10.1002/stem.2974. Epub 2019 Jan 30. PMID: 30681766

Deconstructing Retinal Organoids: Single Cell RNA-Seq Reveals the Cellular Components of Human Pluripotent Stem Cell-Derived Retina. Collin J, Queen R, Zerti D, Dorgau B, Hussain R, Coxhead J, Cockell S, Lako M. Stem Cells. 2019 May;37(5):593-598. doi: 10.1002/stem.2963. Epub 2019 Jan 12. PMID: 30548510

A, Zhu, L, Chichagova V, Basu B, Mozafari-Jovin S et al, Luehrmann R, Grellscheid SN, Johnson CA and Lako M. Splicing factor PRPF31 retinitis pigmentosa (RP11) is caused by disrupted alternative splicing programmes for genes implicated in pre-mRNA splicing, cellular adhesion and ciliogenesis. Nature Communications 2018; 9(1):4234. doi: 10.1038/s41467-018-06448-y. (

Hallam D, Hilgen G, Dorgau B, Zhu L, Yu M, Bojic S, Hewitt P, Schmitt M, Uteng M, Kustermann S, Steel D, Nicholds M, Thomas R, Treumann A, Porter A, Sernagor E, Armstrong L, Lako M. Human-Induced Pluripotent Stem Cells Generate Light Responsive Retinal Organoids with Variable and Nutrient-Dependent Efficiency. Stem Cells. 2018 Jul 13. doi: 10.1002/stem.2883. Stem Cells. 2018 Jul 13. doi: 10.1002/stem.2883.

Special Series: Transplantation of Stem Cells into the Eye. Lako M. Stem Cells. 2018 Aug 22. doi: 10.1002/stem.2896. [Epub ahead of print] No abstract available.

Felemban M, Dorgau B, Hunt NC, Hallam D, Zerti D, Bauer R, Ding Y, Collin J, Steel D, Krasnogor N, Al-Aama J, Lindsay S, Mellough C, Lako M. Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. Acta Biomater. 2018 Jul 1;74:207-221. doi: 10.1016/j.actbio.2018.05.023.

Dorgau B, Felemban M, Sharpe A, Bauer R, Hallam D, Steel DH, Lindsay S, Mellough C, Lako M. Laminin γ3 plays an important role in retinal lamination, photoreceptor organisation and ganglion cell differentiation. Cell Death Dis. 2018 May 23;9(6):615. doi: 10.1038/s41419-018-0648-0.

Chichagova V, Hallam D, Collin J, Zerti D, Dorgau B, Felemban M, Lako M, Steel DH. Cellular regeneration strategies for macular degeneration: past, present and future. Eye (Lond). 2018 May;32(5):946-971. doi: 10.1038/s41433-018-0061-z. Epub 2018 Mar 5. Review.

Hunt NC, Hallam D, Chichagova V, Steel DH, Lako M.Adv Healthc Mater. The Application of Biomaterials to Tissue Engineering Neural Retina and Retinal Pigment Epithelium. 2018 Sep 2:e1800226. doi: 10.1002/adhm.201800226. [Epub ahead of print] Review.

Megaw E, Abu-Arafeh H, Mellough C, Gurniak-Witke C, Witke W, Dhillon B, Wright AF, Lako M and Ffrench-Constant C. (2017) Human induced pluripotent stem cell and animal models of X-linked retinitis pigmentosa identify impaired gelsolin activation as a cause of photoreceptor loss. Nature Communications: 8:271.

Chichagova V, Hallam D, Collin J, Buskin A, Saretzki G, Armstrong L, Yu-Man P, Lako M and Steel D (2017) Human iPSC disease modelling reveals functional and structural defects in retinal pigment epithelial cells harbouring the m.3243A>G mitochondrial DNA mutation. Scientific Reports 26: 12320.

Hallam D, Collin J, Bojic S, Chichagova V, Buskin A, Yu X, Lafage L, Otten EG, Anyfantis G, Mellough C, Przyborski S, Alharthi S, Korolchuk V, Lotery A, Saretzki G, McKibbin M, Armstrong L, Steel D, Lako M. (2017) An iPSC patient specific model of CFH (Y402H) polymorphism displays characteristic features of AMD and indicates a beneficial role for UV light exposure. Stem Cells 35: 2305-2320.

Hunt NC, Hallam D, Karimi A, Mellough CB, Steel DHW and Lako M. (2017) 3D culture of human pluripotent stem cells in RGD - alginate hydrogel improves retinal tissue development. Acta Biomaterilia 49: 329-343..

Zhu L, Gomez A, Saretzki G, Jin S, Tilgner K, Melguzo-Sanchiz D, Anyfantis G, Al-Aama J, Vallier L, Chinnery P, Lako M and Armstrong L (2016) CHCHD2 primes the differentiation potential of human induced pluripotent stem cells to neuroectodermal lineages. J. Cell Biology 215(2):187-202.

Collin J, Mellough CB, Do rgau B, Pryzborki S, Moreno-Gimeno I and Lako M (2016) Using zinc finger nuclease technology to generate CRX-GFP reporter human embryonic stem cells as a tool to identify and study the emergence of photoreceptor precursors during pluripotent stem cell differentiation. Stem Cells 34: 311-21 (Cover page image)

Mellough CB and Lako M (2016) How to build a human. Elife. 24;5. pii: e19826.

Hunt NC and Lako M (2016) Tissue engineering using pluripotent stem cells: multidisciplinary approaches to accelerate bench-to-bedside transition. Regen Med. 6:495-8.

Chichagova V, Sanchez-Vera I, Armstrong L, Steel D, Lako M. (2016) Generation of Human Induced Pluripotent Stem Cells Using RNA-Based Sendai Virus System and Pluripotency Validation of the Resulting Cell Population. Methods Mol Biol. 1353:285-307.

Mellough CB, Collin J, Khazim M, White K, Sernagor E, Steel D and Lako M (2015) IGF-1 signalling plays an important role in the formation of three dimensional laminated neural retina and other ocular structures from human embryonic stem cells. Stem Cells 33:2416-30.

Megaw R, Mellough C, WRIGHT A, Lako M and Ffrench-Constant C (2015) Use of induced pluripotent stem cells technology to understand photoreceptor cytoskeletal dynamics in retinitis pigmentosa. Lancet Suppl 1:S69. doi: 10.1016/S0140-6736(15)60384-1.

Mellough CB, Collin J, Sernagor E, Wride NK, Steel DH and Lako M (2014) Lab generated retina: Realising the dream. Vision Neuroscience 22:1-16.

Mellough CB, Sernagor E, Moreno-Gimeno, I, Steel DHW and Lako M (2012) Efficient stage-specific differentiation of human pluripotent stem cells towards retinal photoreceptor cells. Stem Cells 30:673-86 9.

Armstrong L, Lako M, Buckley N, Lappin TR, Murphy MJ, Nolta JA, Pittenger M, Stojkovic M (2012). Editorial: Our top 10 developments in stem cell biology over the last 30 years. Stem Cells. 30:2-9.

Collin J, Lako M. (2011) Concise review: putting a finger on stem cell biology: zinc finger nuclease-driven targeted genetic editing in human pluripotent stem cells. Stem Cells. 29:1021-33.

Armstrong L, Tilgner K, Saretzki G, Atkinson SP, Stojkovic M, Moreno R, Przyborski S, Lako M.(2010) Human induced pluripotent stem cell lines show stress defense mechanisms and mitochondrial regulation similar to those of human embryonic stem cells. Stem Cells. 28:661-73.

Lako M, Armstrong L, Stojkovic M (2010). Induced pluripotent stem cells : it looks simple but can looks deceive? Stem Cells.28:845-50.

Mellough CB, Steel DH, Lako M. (2009) Genetic basis of inherited macular dystrophies and implications for stem cell therapy. Stem Cells. 27(11):2833-45.

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